Bruna Alice Gomes de Melo

@article{Romariz2024,

title = {High Concentrations of Cannabidiol Induce Neurotoxicity in Neurosphere Culture System},

author = {Simone A. A. Romariz and Viviam Sanabria and Karina Ribeiro da Silva and Miguel L. Quintella and Bruna A. G. de Melo and Marimélia Porcionatto and Danilo Candido de Almeida and Beatriz M. Longo},

doi = {10.1007/s12640-024-00692-5},

issn = {1476-3524},

year  = {2024},

date = {2024-02-00},

journal = {Neurotox Res},

volume = {42},

number = {1},

publisher = {Springer Science and Business Media LLC},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{deMelo2022,

title = {3D Bioprinted Neural‐Like Tissue as a Platform to Study Neurotropism of Mouse‐Adapted SARS‐CoV‐2},

author = {Bruna A. G. de Melo and Mayara V. Mundim and Robertha M. R. Lemes and Elisa M. Cruz and Tais N. Ribeiro and Carolina F. Santiago and Jéssica H. L. da Fonsêca and Julia C. Benincasa and Roberta S. Stilhano and Nathalia Mantovani and Luiz C. Santana and Ricardo Durães‐Carvalho and Ricardo S. Diaz and Luiz M. R. Janini and Juliana T. Maricato and Marimelia A. Porcionatto},

doi = {10.1002/adbi.202200002},

issn = {2701-0198},

year  = {2022},

date = {2022-08-00},

journal = {Advanced Biology},

volume = {6},

number = {8},

publisher = {Wiley},

abstract = {AbstractThe effects of neuroinvasion by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) become clinically relevant due to the numerous neurological symptoms observed in Corona Virus Disease 2019 (COVID‐19) patients during infection and post‐COVID syndrome or long COVID. This study reports the biofabrication of a 3D bioprinted neural‐like tissue as a proof‐of‐concept platform for a more representative study of SARS‐CoV‐2 brain infection. Bioink is optimized regarding its biophysical properties and is mixed with murine neural cells to construct a 3D model of COVID‐19 infection. Aiming to increase the specificity to murine cells, SARS‐CoV‐2 is mouse‐adapted (MA‐SARS‐CoV‐2) in vitro, in a protocol first reported here. MA‐SARS‐CoV‐2 reveals mutations located at the Orf1a and Orf3a domains and is evolutionarily closer to the original Wuhan SARS‐CoV‐2 strain than SARS‐CoV‐2 used for adaptation. Remarkably, MA‐SARS‐CoV‐2 shows high specificity to murine cells, which present distinct responses when cultured in 2D and 3D systems, regarding cell morphology, neuroinflammation, and virus titration. MA‐SARS‐CoV‐2 represents a valuable tool in studies using animal models, and the 3D neural‐like tissue serves as a powerful in vitro platform for modeling brain infection, contributing to the development of antivirals and new treatments for COVID‐19.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Mehrotra2022,

title = {Mimicking Native Heart Tissue Physiology and Pathology in Silk Fibroin Constructs through a Perfusion‐Based Dynamic Mechanical Stimulation Microdevice},

author = {Shreya Mehrotra and Bruna Alice Gomes de Melo and Mario Miscuglio and Kiavash Kiaee and Su Ryon Shin and Biman B. Mandal},

doi = {10.1002/adhm.202101678},

issn = {2192-2659},

year  = {2022},

date = {2022-03-00},

journal = {Adv Healthcare Materials},

volume = {11},

number = {6},

publisher = {Wiley},

abstract = {AbstractIn vitro cardiomyocyte (CM) maturation is an imperative step to replicate native heart tissue‐like structures as cardiac tissue grafts or as drug screening platforms. CMs are known to interpret biophysical cues such as stiffness, topography, external mechanical stimulation or dynamic perfusion load through mechanotransduction and change their behavior, organization, and maturation. In this regard, a silk‐based cardiac tissue (CT) coupled with a dynamic perfusion‐based mechanical stimulation platform (DMM) for achieving maturation and functionality in vitro is tried to be delivered. Silk fibroin (SF) is used to fabricate lamellar scaffolds to provide native tissue‐like anisotropic architecture and is found to be nonimmunogenic and biocompatible allowing cardiomyocyte attachment and growth in vitro. Further, the scaffolds display excellent mechanical properties by their ability to undergo cyclic compressions without any deformation when places in the DMM. Gradient compression strains (5% to 20%), mimicking the native physiological and pathological conditions, are applied to the cardiomyocyte culture seeded on lamellar silk scaffolds in the DMM. A strain‐dependent difference in cardiomyocyte maturation, gene expression, sarcomere elongation, and extracellular matrix formation is observed. These silk‐based CTs matured in the DMM can open up several avenues toward the development of host‐specific grafts and in vitro models for drug screening.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{deMelo2021,

title = {3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue},

author = {Bruna A. G. de Melo and Elisa M. Cruz and Taís N. Ribeiro and Mayara V. Mundim and Marimelia A. Porcionatto},

doi = {10.3791/62691},

issn = {1940-087X},

year  = {2021},

date = {2021-00-00},

journal = {JoVE},

number = {173},

publisher = {MyJove Corporation},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Cavalcanti2020,

title = {Performance of the main downstream operations on hyaluronic acid purification},

author = {André Delano Domingos Cavalcanti and Bruna Alice Gomes de Melo and Bruno Armenio Moreira Ferreira and Maria Helena Andrade Santana},

doi = {10.1016/j.procbio.2020.08.020},

issn = {1359-5113},

year  = {2020},

date = {2020-12-00},

journal = {Process Biochemistry},

volume = {99},

pages = {160--170},

publisher = {Elsevier BV},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{deMelo2020b,

title = {Strategies to use fibrinogen as bioink for 3D bioprinting fibrin-based soft and hard tissues},

author = {Bruna A.G. de Melo and Yasamin A. Jodat and Elisa M. Cruz and Julia C. Benincasa and Su Ryon Shin and Marimelia A. Porcionatto},

doi = {10.1016/j.actbio.2020.09.024},

issn = {1742-7061},

year  = {2020},

date = {2020-11-00},

journal = {Acta Biomaterialia},

volume = {117},

pages = {60--76},

publisher = {Elsevier BV},

keywords = {},

pubstate = {published},

tppubtype = {article}

}