Rosana Almada Bassani

@article{nokey,

title = {BugHeart: software for online monitoring and quantitation of contractile activity of the insect heart},

author = {Arnaldo Fim Neto and Rosana Almada Bassani and Pedro X de Oliveira and José Wilson Magalhães Bassani},

doi = {https://doi.org/10.1007/s42600-019-00026-x},

year  = {2019},

date = {2019-11-09},

urldate = {2019-11-09},

journal = {Research on Biomedical Engineering },

volume = {35},

pages = {235–240},

abstract = {The insect heart (dorsal vessel, DV) is considered a valuable model for studies on cardiac genetics, development, and physiology. However, as software for monitoring and quantitation of insect cardiac activity is not commercially available, most studies depend on time-consuming, post hoc analysis of video records. In this study, a computer program (BugHeart) was developed for this purpose, and applied to the determination of the octopamine effects on Tenebrio molitor DV.



Methods

The software was developed in Labview 11.0 for online processing of amplified video images of the transilluminated DV, in which systolic variation of the luminal diameter can be monitored over successive contraction cycles by video-tracking the tube inner edge. The possibility of adjustment of light intensity threshold and the introduction of calibration allow online quantitation of the DV luminal diameter and its cyclic variation (contraction amplitude), as well as heart rate (HR) estimation. The program can export video and text files for documentation and further analysis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid31084419,

title = {High Thyrotropin Is Critical for Cardiac Electrical Remodeling and Arrhythmia Vulnerability in Hypothyroidism},

author = {Julieta Fernandez-Ruocco and Monica Gallego and Ainhoa Rodriguez-de-Yurre and Julian Zayas-Arrabal and Leyre Echeazarra and Amaia Alquiza and Victor Fernández-López and Juan M Rodriguez-Robledo and Oscar Brito and Ygor Schleier and Marisa Sepulveda and Natália Ferreira Oshiyama and Martin Vila-Petroff and Rosana Almada Bassani and Emiliano H Medei and Oscar Casis},

doi = {10.1089/thy.2018.0709},

issn = {1557-9077},

year  = {2019},

date = {2019-07-01},

urldate = {2019-07-01},

journal = {Thyroid},

volume = {29},

number = {7},

pages = {934--945},

abstract = { Hypothyroidism, the most common endocrine disease, induces cardiac electrical remodeling that creates a substrate for ventricular arrhythmias. Recent studies report that high thyrotropin (TSH) levels are related to cardiac electrical abnormalities and increased mortality rates. The aim of the present work was to investigate the direct effects of TSH on the heart and its possible causative role in the increased incidence of arrhythmia in hypothyroidism.  A new rat model of central hypothyroidism (low TSH levels) was created and characterized together with the classical propylthiouracil-induced primary hypothyroidism model (high TSH levels). Electrocardiograms were recorded , and ionic currents were recorded from isolated ventricular myocytes  by the patch-clamp technique. Protein and mRNA were measured by Western blot and quantitative reverse transcription polymerase chain reaction in rat and human cardiac myocytes. Adult human action potentials were simulated  to incorporate the experimentally observed changes.  Both primary and central hypothyroidism models increased the L-type Ca current (I) and decreased the ultra-rapid delayed rectifier K current (I) densities. However, only primary but not central hypothyroidism showed electrocardiographic repolarization abnormalities and increased ventricular arrhythmia incidence during caffeine/dobutamine challenge. These changes were paralleled by a decrease in the density of the transient outward K current (I) in cardiomyocytes from animals with primary but not central hypothyroidism.  treatment with TSH for 24 hours enhanced isoproterenol-induced spontaneous activity in control ventricular cells and diminished I density in cardiomyocytes from control and central but not primary hypothyroidism animals. In human myocytes, TSH decreased the expression of  and , I, and the delayed rectifier K current (I) encoding proteins in a protein kinase A-dependent way. Transposing the changes produced by hypothyroidism and TSH to a computer model of human ventricular action potential resulted in enhanced occurrence of early afterdepolarizations and arrhythmia mostly in primary hypothyroidism, especially under β-adrenergic stimulation.  The results suggest that suppression of repolarizing K currents by TSH underlies most of the electrical remodeling observed in hypothyroidism. This work demonstrates that the activation of the TSH-receptor/protein kinase A pathway in the heart is responsible for the cardiac electrical remodeling and arrhythmia generation seen in hypothyroidism.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid30218147,

title = {Sources of Ca for contraction of the heart tube of Tenebrio molitor (Coleoptera: Tenebrionidae)},

author = {Arnaldo Fim Neto and Rosana Almada Bassani and Pedro X de Oliveira and José Wilson Magalhães Bassani},

doi = {10.1007/s00360-018-1183-0},

issn = {1432-136X},

year  = {2018},

date = {2018-11-01},

urldate = {2018-11-01},

journal = {J Comp Physiol B},

volume = {188},

number = {6},

pages = {929--937},

abstract = {Insect and vertebrate hearts share the ability to generate spontaneously their rhythmic electrical activity, which triggers the fluid-propelling mechanical activity. Although insects have been used as models in studies on the impact of genetic alterations on cardiac function, there is surprisingly little information on the generation of the inotropic activity in their hearts. The main goal of this study was to investigate the sources of Ca for contraction in Tenebrio molitor hearts perfused in situ, in which inotropic activity was assessed by the systolic variation of the cardiac luminal diameter. Increasing the pacing rate from 1.0 to 2.5 Hz depressed contraction amplitude and accelerated relaxation. To avoid inotropic interference of variations in spontaneous rate, which have been shown to occur in insect heart during maneuvers that affect Ca cycling, experiments were performed under electrical pacing at near-physiological rates. Raising the extracellular Ca concentration from 0.5 to 8 mM increased contraction amplitude in a manner sensitive to L-type Ca channel blockade by D600. Inotropic depression was observed after treatment with caffeine or thapsigargin, which impair Ca accumulation by the sarcoplasmic reticulum (SR). D600, but not inhibition of the sarcolemmal Na/Ca exchanger by KB-R7943, further depressed inotropic activity in thapsigargin-treated hearts. From these results, it is possible to conclude that in T. molitor heart, as in vertebrates: (a) inotropic and lusitropic activities are modulated by the heart rate; and (b) Ca availability for contraction depends on both Ca influx via L-type channels and Ca release from the SR.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid25395252,

title = {Electrocardiogram, heart movement and heart rate in the awake gecko (Hemidactylus mabouia)},

author = {Carina M Germer and Juliana M Tomaz and Ana F Carvalho and Rosana A Bassani and José W M Bassani},

doi = {10.1007/s00360-014-0873-5},

issn = {1432-136X},

year  = {2015},

date = {2015-01-01},

journal = {J Comp Physiol B},

volume = {185},

number = {1},

pages = {111--118},

abstract = {The electrocardiogram (ECG) is the simplest and most effective non-invasive method to assess the electrical activity of the heart and to obtain information on the heart rate (HR) and rhythm. Because information on the HR of very small reptiles (body mass <10 g) is still scarce in the literature, in the present work we describe a procedure for recording the ECG in non-anesthetized geckos (Hemidactylus mabouia, Moreau de Jonnès, 1818) under different conditions, namely manual restraint (MR), spontaneous tonic immobility (TI), and in the non-restrained condition (NR). In the gecko ECG, the P, QRS and T waves were clearly distinguishable. The HR was 2.83 ± 0.02 Hz under MR, which was significantly greater (p < 0.001) than the HR under the TI (1.65 ± 0.09 Hz) and NR (1.60 ± 0.10 Hz) conditions. Spontaneously beating isolated gecko hearts contracted at 0.84 ± 0.03 Hz. The in vitro beating rate was affected in a concentration-dependent fashion by adrenoceptor stimulation with noradrenaline, as well as by the muscarinic cholinergic agonist carbachol, which produced significant positive and negative chronotropic effects, respectively (p < 0.001). To our knowledge, this is the first report on the ECG morphology and HR values in geckos, particularly under TI. The methodology and instrumentation developed here are useful for non-invasive in vivo physiological and pharmacological studies in small reptiles without the need of physical restraint or anesthesia.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Medical equipment classification according to corrective maintenance data: a strategy based on the equipment age},

author = {Natália Ferreira Oshiyama and Ana Carolina Silveira and Rosana Almada Bassani and José Wilson Magalhães Bassani},

doi = {https://doi.org/10.4322/rbeb.2013.045},

year  = {2014},

date = {2014-04-23},

journal = {Rev. Bras. Eng. Bioméd},

volume = {30},

issue = {1},

abstract = {Decision-making on medical equipment management is a daily task for clinical engineers, but it may prove difficult to easily extract relevant information from the large amount of data from computerized maintenance management systems. This article describes a simple method of medical equipment classification based on corrective maintenance indicators. METHODS: Three indicators were calculated based on the number of events, duration and cost of corrective maintenance. Three classes were defined according to the indicator values of different equipment ages: class A for 0-4 years, class B for 5-9 years, and class C for equipment older than 10 years. The method was applied to 2,134 pieces of equipment from the Health Service system of the University of Campinas. RESULTS: From the total, 51.7% of the equipment were classified as C, 4.2% as B and 44.1% as A. The infusion pump for general use was the type of equipment of which most units were in the C class (84.7%), even though almost 50% of them were acquired within less than 9 years, and would thus be expected to be classified as A and B. Among the pumps in class C, 39.5% were from a single manufacturer, although the equipments were acquired recently. CONCLUSION: The developed classification may be an important tool for raising alerts about equipment more prone to maintenance problems, as well as for identification of equipments with acceptable maintenance history, supporting decision-making on equipment replacement.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24140800,

title = {Muscarinic stimulation and pinacidil produce similar facilitation of tachyarrhythmia induction in rat isolated atria},

author = {Nivaldo Zafalon and Natália Ferreira Oshiyama and José Wilson Magalhães Bassani and Rosana Almada Bassani},

doi = {10.1016/j.yjmcc.2013.10.004},

issn = {1095-8584},

year  = {2013},

date = {2013-12-01},

urldate = {2013-12-01},

journal = {J Mol Cell Cardiol},

volume = {65},

pages = {120--126},

abstract = {Atrial tachyarrhythmias, the most common type of cardiac arrhythmias, are associated with greater stroke risk. Muscarinic cholinergic agonists have been shown to facilitate atrial tachyarrhythmia maintenance in the absence of cardiac disease. This has been attributed to action potential shortening, which enhances myocardial electrical anisotropy, and thus creates a substrate for reentrant excitation. In this study, we describe a similar effect of the ATP-sensitive K(+) channel (KATP) opener pinacidil on tachyarrhythmia induction in isolated rat atria. Pinacidil, which activates a weakly inwardly-rectifying current in isolated atrial myocytes, enhanced arrhythmia induction in the right and left atria. This effect was abolished by the KATP blocker glibenclamide, but not by atropine, which rules out a possible indirect effect due to stimulation of acetylcholine release. However, pinacidil attenuated carbachol-induced tachyarrhythmia facilitation, which may indicate that the action of these agonists converges to a common cellular mechanism. Both agonists caused marked action potential shortening in isolated atrial myocytes. Moreover, during arrhythmia in the presence of pinacidil and carbachol, the atrial vectorelectrographic patterns were similar and consistent with reentrant propagation of the electrical activity. From these results, we conclude that the KATP channel opening is pro-arrhythmic in atrial tissue, which may pose as an additional risk in the scenario of myocardial hypoxia. Moreover, the similarity of the electrophysiological effects of pinacidil and carbachol is suggestive that the sole increase in background K(+) conductance is sufficient for atrial tachyarrhythmia facilitation.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}