Publicações

@article{Alegria2021,

title = {Causal regression for online estimation of highly nonlinear parametrically varying models},

author = {Elvis Jara Alegria and Mateus Giesbrecht and Celso Pascoli Bottura},

doi = {10.1016/j.automatica.2020.109425},

issn = {0005-1098},

year  = {2021},

date = {2021-03-00},

journal = {Automatica},

volume = {125},

publisher = {Elsevier BV},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Marinho2021,

title = {Application of differential evolution to multi-objective tuning of vibration spectrum analyzers based on microelectromechanical systems},

author = {Yara Quilles Marinho and Fabiano Fruett and Mateus Giesbrecht},

doi = {10.1016/j.engappai.2020.104071},

issn = {0952-1976},

year  = {2021},

date = {2021-01-00},

journal = {Engineering Applications of Artificial Intelligence},

volume = {97},

publisher = {Elsevier BV},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{deMelo2021,

title = {3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue},

author = {Bruna A. G. de Melo and Elisa M. Cruz and Taís N. Ribeiro and Mayara V. Mundim and Marimelia A. Porcionatto},

doi = {10.3791/62691},

issn = {1940-087X},

year  = {2021},

date = {2021-00-00},

journal = {JoVE},

number = {173},

publisher = {MyJove Corporation},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Cavalcanti2020,

title = {Performance of the main downstream operations on hyaluronic acid purification},

author = {André Delano Domingos Cavalcanti and Bruna Alice Gomes de Melo and Bruno Armenio Moreira Ferreira and Maria Helena Andrade Santana},

doi = {10.1016/j.procbio.2020.08.020},

issn = {1359-5113},

year  = {2020},

date = {2020-12-00},

journal = {Process Biochemistry},

volume = {99},

pages = {160--170},

publisher = {Elsevier BV},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{deMelo2020b,

title = {Strategies to use fibrinogen as bioink for 3D bioprinting fibrin-based soft and hard tissues},

author = {Bruna A.G. de Melo and Yasamin A. Jodat and Elisa M. Cruz and Julia C. Benincasa and Su Ryon Shin and Marimelia A. Porcionatto},

doi = {10.1016/j.actbio.2020.09.024},

issn = {1742-7061},

year  = {2020},

date = {2020-11-00},

journal = {Acta Biomaterialia},

volume = {117},

pages = {60--76},

publisher = {Elsevier BV},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dosSantosPedotti2020,

title = {Low-cost MEMS accelerometer network for rotating machine vibration diagnostics},

author = {Luciane Agnoletti dos Santos Pedotti and Ricardo Mazza Zago and Mateus Giesbrecht and Fabiano Fruett},

doi = {10.1109/mim.2020.9234762},

issn = {1941-0123},

year  = {2020},

date = {2020-10-00},

journal = {IEEE Instrum. Meas. Mag.},

volume = {23},

number = {7},

pages = {25--33},

publisher = {Institute of Electrical and Electronics Engineers (IEEE)},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid32058446,

title = {Computed tomography-based skeletal segmentation for quantitative PET metrics of bone involvement in multiple myeloma},

author = {Maria Emília Seren Takahashi and Camila Mosci and Edna Marina de Souza and Sergio Querino Brunetto and Cármino de Souza and Fernando Vieira Pericole and Irene Lorand-Metze and Celso Dario Ramos},

doi = {10.1097/MNM.0000000000001165},

issn = {1473-5628},

year  = {2020},

date = {2020-04-01},

urldate = {2020-04-01},

journal = {Nucl Med Commun},

volume = {41},

number = {4},

pages = {377--382},

abstract = {PURPOSE: Quantifications in nuclear medicine are occasionally limited by the lack of standardization for defining volumes of interest (VOIs) on functional images. In the present article, we propose the use of computed tomography (CT)-based skeletal segmentation to determine anatomically the VOI in order to calculate quantitative parameters of fluorine 18 fluorodeoxyglucose (F-FDG) PET/CT images from patients with multiple myeloma.



METHODS: We evaluated 101 whole-body F-FDG PET/CTs of 58 patients with multiple myeloma. An initial subjective visual analysis of the PET images was used to classify the bone involvement as negative/mild, moderate, or marked. Then, a fully automated CT-based segmentation of the skeleton was performed on PET images. The maximum, mean, and SD of the standardized uptake values (SUVmax, SUVmean, and SDSUV) were calculated for bone tissue and compared with the visual analysis.



RESULTS: Forty-five (44.5%), 32 (31.7%), and 24 (23.8%) PET images were, respectively, classified as negative/mild, moderate, or marked bone involvement. All quantitative parameters were significantly related to the visual assessment of bone involvement. This association was stronger for the SUVmean [odds ratio (OR): 10.52 (95% confidence interval (CI), 5.68-19.48); P < 0.0001] and for the SDSUV [OR: 5.58 (95% CI, 3.31-9.42); P < 0.001) than for the SUVmax [OR: 1.01 (95% CI, 1.003-1.022); P = 0.003].



CONCLUSION: CT-based skeletal segmentation allows for automated and therefore reproducible calculation of PET quantitative parameters of bone involvement in patients with multiple myeloma. Using this method, the SUVmean and its respective SD correlated better with the visual analysis of F-FDG PET images than SUVmax. Its value in staging and evaluating therapy response needs to be evaluated.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid32120053,

title = {Reliable measurement of incisal bite force for understanding the control of masticatory muscles},

author = {Luciana S Moreira and Leonardo A Elias and Carina M Germer and Evanisi T Palomari},

doi = {10.1016/j.archoralbio.2020.104683},

issn = {1879-1506},

year  = {2020},

date = {2020-04-01},

urldate = {2020-04-01},

journal = {Arch Oral Biol},

volume = {112},

pages = {104683},

abstract = {OBJECTIVE: In the present study, we aimed at evaluating the steadiness of incisal bite force during isometric contractions of masticatory muscles.nnDESIGN: Two separate experiments were carried out in 11 healthy young women. A first experiment was performed to test the reliability of our protocol for measurement of incisal bite force steadiness. The second experiment aimed to evaluate the steadiness of incisal bite force at four submaximal (i.e., percentage of maximum voluntary contraction, MVC) levels (5 %MVC, 10 %MVC, 15 %MVC, and 20 %MVC), along with the bilateral myoelectric activity of two masticatory muscles (temporalis and masseter).nnRESULTS: The results from the first experiment showed that our protocol is substantially reliable (intraclass correlation coefficient, ICC > 0.80) for estimating force variability and moderate reliable (0.60 < ICC < 0.80) for estimating spectral properties of force signals. In the second experiment, we found that force standard deviation (SD) increased proportionally to the power of mean force, and coefficient of variation (CoV) was higher at low-intensity contractions and maintained at an approximately constant level for high-intensity contractions. The force-EMG relationships were linear for both muscles at the contraction intensities evaluated in the study (5 %MVC to 20 %MVC), and the median frequency did not change with contraction intensity.nnCONCLUSION: Therefore, we presented a reliable method to estimate the incisal bite force, along with additional data on force control and myoelectric activity of jaw elevator muscles during isometric steady contractions.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{deMelo2020,

title = {Hyaluronic acid and fibrin from L-PRP form semi-IPNs with tunable properties suitable for use in regenerative medicine},

author = {Bruna Alice Gomes de Melo and Carla Giometti França and José Luis Dávila and Nilza Alzira Batista and Carolina Caliari-Oliveira and Marcos Akira d'Ávila and Ângela Cristina Malheiros Luzo and José Fabio Santos Duarte Lana and Maria Helena Andrade Santana},

doi = {10.1016/j.msec.2019.110547},

issn = {0928-4931},

year  = {2020},

date = {2020-04-00},

journal = {Materials Science and Engineering: C},

volume = {109},

publisher = {Elsevier BV},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Impact of voltage-gated Na + channel biophysical properties on action potential upstroke},

author = {Natália Ferreira Oshiyama and Rosana Almada Bassani and José Wilson Magalhães Bassani},

doi = {https://doi.org/10.1016/j.yjmcc.2019.11.116},

year  = {2020},

date = {2020-03-01},

volume = {140},

pages = {48-49},

abstract = {The main determinant of conduction velocity is the AP maximum depolarization rate (dEm/dtmax), which depends on the voltage-gated Na+ channels biophysical properties. Here we investigated age-dependent differences in whole-cell Na+ current (INa) and dEm/dtmax in myocytes isolated from neonatal and adult Wistar rats. The impact of Na+ channel biophysical properties on AP overshoot was evaluated with an AP model developed with measured whole-cell INa, Ca2+, and transient outward and delayed rectifier K+ currents from immature cells, described according to Hodgkin-Huxley kinetics. Even though INa density was 2-fold greater in neonatal myocytes (−71.9 ± 35.5, n = 11; 33.3 ± 1 6.7 pA/pF in adults, n = 6; p < .01), dEm/dtmax was not, and tended to be lower in neonates (84.2 ± 35.8 V/s, n = 12) than in adults (100.3 ± 44.4 V/s, n = 7; p = .40). The half-maximal activation voltage (E1/2) was less negative (−31.5 ± 1.7 mV vs. -44.4 ± 6.0 mV; p < .001), and the activation curve had greater slope (k: 7.8 ± 1.2 mV vs. 4.4 ± 0.6 mV; p < .001) in immature than in adult cells. A positive shift was also observed in the steady-state inactivation curve in neonates (E1/2: −76.2 ± 7.8 mV vs. -85.6 ± 4.1 mV; p < .05). The dEm/dtmax of the simulated AP in neonatal myocyte was 81 V/s. However, when the activation and inactivation equations were adjusted to reproduce E1/2 and k values estimated in adult cells, dEm/dtmax increased to 96 V/s, close to the experimental value in adults. Therefore, it seems that, in addition to INa density, voltage-dependence of Na+ channel activation and inactivation may exert marked influence on depolarization rate, and AP propagation velocity.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Impact of voltage-gated Na + channel biophysical properties on action potential upstroke},

author = {Natália Ferreira Oshiyama and Rosana Almada Bassani and José Wilson Magalhães Bassani},

doi = {https://doi.org/10.1016/j.yjmcc.2019.11.116},

year  = {2020},

date = {2020-03-01},

urldate = {2020-03-01},

journal = {Journal of Molecular and Cellular Cardiology},

volume = {140},

pages = {48-49},

abstract = {The main determinant of conduction velocity is the AP maximum depolarization rate (dEm/dtmax), which depends on the voltage-gated Na+ channels biophysical properties. Here we investigated age-dependent differences in whole-cell Na+ current (INa) and dEm/dtmax in myocytes isolated from neonatal and adult Wistar rats. The impact of Na+ channel biophysical properties on AP overshoot was evaluated with an AP model developed with measured whole-cell INa, Ca2+, and transient outward and delayed rectifier K+ currents from immature cells, described according to Hodgkin-Huxley kinetics. Even though INa density was 2-fold greater in neonatal myocytes (−71.9 ± 35.5, n = 11; 33.3 ± 1 6.7 pA/pF in adults, n = 6; p < .01), dEm/dtmax was not, and tended to be lower in neonates (84.2 ± 35.8 V/s, n = 12) than in adults (100.3 ± 44.4 V/s, n = 7; p = .40). The half-maximal activation voltage (E1/2) was less negative (−31.5 ± 1.7 mV vs. -44.4 ± 6.0 mV; p < .001), and the activation curve had greater slope (k: 7.8 ± 1.2 mV vs. 4.4 ± 0.6 mV; p < .001) in immature than in adult cells. A positive shift was also observed in the steady-state inactivation curve in neonates (E1/2: −76.2 ± 7.8 mV vs. -85.6 ± 4.1 mV; p < .05). The dEm/dtmax of the simulated AP in neonatal myocyte was 81 V/s. However, when the activation and inactivation equations were adjusted to reproduce E1/2 and k values estimated in adult cells, dEm/dtmax increased to 96 V/s, close to the experimental value in adults. Therefore, it seems that, in addition to INa density, voltage-dependence of Na+ channel activation and inactivation may exert marked influence on depolarization rate, and AP propagation velocity.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Jodat2020,

title = {A 3D‐Printed Hybrid Nasal Cartilage with Functional Electronic Olfaction},

author = {Yasamin A. Jodat and Kiavash Kiaee and Daniel Vela Jarquin and Rosakaren Ludivina De la Garza Hernández and Ting Wang and Sudeep Joshi and Zahra Rezaei and Bruna Alice Gomes de Melo and David Ge and Manu S. Mannoor and Su Ryon Shin},

doi = {10.1002/advs.201901878},

issn = {2198-3844},

year  = {2020},

date = {2020-03-00},

journal = {Advanced Science},

volume = {7},

number = {5},

publisher = {Wiley},

abstract = {AbstractAdvances in biomanufacturing techniques have opened the doors to recapitulate human sensory organs such as the nose and ear in vitro with adequate levels of functionality. Such advancements have enabled simultaneous targeting of two challenges in engineered sensory organs, especially the nose: i) mechanically robust reconstruction of the nasal cartilage with high precision and ii) replication of the nose functionality: odor perception. Hybrid nasal organs can be equipped with remarkable capabilities such as augmented olfactory perception. Herein, a proof‐of‐concept for an odor‐perceptive nose‐like hybrid, which is composed of a mechanically robust cartilage‐like construct and a biocompatible biosensing platform, is proposed. Specifically, 3D cartilage‐like tissue constructs are created by multi‐material 3D bioprinting using mechanically tunable chondrocyte‐laden bioinks. In addition, by optimizing the composition of stiff and soft bioinks in macro‐scale printed constructs, the competence of this system in providing improved viability and recapitulation of chondrocyte cell behavior in mechanically robust 3D constructs is demonstrated. Furthermore, the engineered cartilage‐like tissue construct is integrated with an electrochemical biosensing system to bring functional olfactory sensations toward multiple specific airway disease biomarkers, explosives, and toxins under biocompatible conditions. Proposed hybrid constructs can lay the groundwork for functional bionic interfaces and humanoid cyborgs.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Mehrotra2020,

title = {Nonmulberry Silk Based Ink for Fabricating Mechanically Robust Cardiac Patches and Endothelialized Myocardium‐on‐a‐Chip Application},

author = {Shreya Mehrotra and Bruna A. G. de Melo and Minoru Hirano and Wendy Keung and Ronald A. Li and Biman B. Mandal and Su Ryon Shin},

doi = {10.1002/adfm.201907436},

issn = {1616-3028},

year  = {2020},

date = {2020-03-00},

journal = {Adv Funct Materials},

volume = {30},

number = {12},

publisher = {Wiley},

abstract = {AbstractBioprinting holds great promise toward engineering functional cardiac tissue constructs for regenerative medicine and as drug test models. However, it is highly limited by the choice of inks that require maintaining a balance between the structure and functional properties associated with the cardiac tissue. In this regard, a novel and mechanically robust biomaterial‐ink based on nonmulberry silk fibroin protein is developed. The silk‐based ink demonstrates suitable mechanical properties required in terms of elasticity and stiffness (≈40 kPa) for developing clinically relevant cardiac tissue constructs. The ink allows the fabrication of stable anisotropic scaffolds using a dual crosslinking method, which are able to support formation of aligned sarcomeres, high expression of gap junction proteins as connexin‐43, and maintain synchronously beating of cardiomyocytes. The printed constructs are found to be nonimmunogenic in vitro and in vivo. Furthermore, delving into an innovative method for fabricating a vascularized myocardial tissue‐on‐a‐chip, the silk‐based ink is used as supporting hydrogel for encapsulating human induced pluripotent stem cell derived cardiac spheroids (hiPSC‐CSs) and creating perfusable vascularized channels via an embedded bioprinting technique. The ability is confirmed of silk‐based supporting hydrogel toward maturation and viability of hiPSC‐CSs and endothelial cells, and for applications in evaluating drug toxicity.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid31791034,

title = {Neurophysiological correlates of force control improvement induced by sinusoidal vibrotactile stimulation},

author = {Carina Marconi Germer and Alessandro Del Vecchio and Francesco Negro and Dario Farina and Leonardo Abdala Elias},

doi = {10.1088/1741-2552/ab5e08},

issn = {1741-2552},

year  = {2020},

date = {2020-01-01},

journal = {J Neural Eng},

volume = {17},

number = {1},

pages = {016043},

abstract = {OBJECTIVE: An optimal level of vibrotactile stimulation has been shown to improve sensorimotor control in healthy and diseased individuals. However, the underlying neurophysiological mechanisms behind the enhanced motor performance caused by vibrotactile stimulation are yet to be fully understood. Therefore, here we aim to evaluate the effect of a cutaneous vibration on the firing behavior of motor units in a condition of improved force steadiness.nnAPPROACH: Participants performed a visuomotor task, which consisted of low-intensity isometric contractions of the first dorsal interosseous (FDI) muscle, while sinusoidal (175 Hz) vibrotactile stimuli with different intensities were applied to the index finger. High-density surface electromyogram was recorded from the FDI muscle, and a decomposition algorithm was used to extract the motor unit spike trains. Additionally, computer simulations were performed using a multiscale neuromuscular model to provide a potential explanation for the experimental findings.nnMAIN RESULTS: Experimental outcomes showed that an optimal level of vibration significantly improved force steadiness (estimated as the coefficient of variation of force). The decreased force variability was accompanied by a reduction in the variability of the smoothed cumulative spike train (as an estimation of the neural drive to the muscle), and the proportion of common inputs to the FDI motor nucleus. However, the interspike interval variability did not change significantly with the vibration. A mathematical approach, together with computer simulation results suggested that vibrotactile stimulation would reduce the variance of the common synaptic input to the motor neuron pool, thereby decreasing the low frequency fluctuations of the neural drive to the muscle and force steadiness.nnSIGNIFICANCE: Our results demonstrate that the decreased variability in common input accounts for the enhancement in force control induced by vibrotactile stimulation.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid32547479,

title = {Clinical Usefulness of SISCOM-SPM Compared to Visual Analysis to Locate the Epileptogenic Zone},

author = {Carla Oliveira Young and Elba C S C Etchbehere and Edna Marina de Souza and Sergio Querino Brunetto and Allan de Oliveira Santos and Mariana C L Lima and Sebastian Ortiz-De la Rosa and Marina Alvim and Clarissa Lin Yasuda and Celso Darío Ramos and Fernando Cendes and Bárbara Juarez Amorim},

doi = {10.3389/fneur.2020.00467},

issn = {1664-2295},

year  = {2020},

date = {2020-01-01},

urldate = {2020-01-01},

journal = {Front Neurol},

volume = {11},

pages = {467},

abstract = { Subtraction of ictal-interictal SPECT co-registered to MRI (SISCOM) is a quantification tool that can improve the sensitivity and specificity of the epileptogenic zone (EZ) localization. Commercially available image analysis software packages for SISCOM are costly, and Statistical Parametric Mapping (SPM) could be an alternative free software for the definition of the EZ. There are only a few studies that compare SISCOM using SPM (SISCOM-SPM) with visual analysis.  To compare SISCOM-SPM vs. visual analysis for localization of the EZ in patients with pharmacoresistant focal epilepsies.  We evaluated all our patients with focal epilepsies that underwent ictal and interictal SPECT. We defined the reference standard to locate the EZ by pathology and follow-up (in patients submitted to surgery), or seizure semiology, serial EEG, long-term video-EEG, F-FDG PET/CT, and MRI (in patients who were not operated). We compared the location of the EZ by visual analysis of SPECT images and by SISCOM-SPM to the reference standard and classified as , or .  We included 23 patients. Visual analysis was  with the EZ reference standard in only 13 patients (56.5%), while SISCOM-SPM was  in 18 cases (78.3%), providing a 21.8% increase in the location of EZ. However, this difference was not significant due to the small sample size ( = 0.0856).  Our preliminary results demonstrate that, in clinical practice, SISCOM-SPM has the potential to add information that might help localize the EZ compared to visual analysis. SISCOM-SPM has a lower cost than other commercially available SISCOM software packages, which is an advantage for developing countries. Studies with more patients are necessary to confirm our findings.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}